TY - JOUR
T1 - Quantitative Peptidomic profiling of caprine and bovine Milk after simulated gastrointestinal digestion
T2 - A multidimensional analytical strategy integrating UHPLC-Q-TOF-MS and QqQ-MS
AU - Zhao, Fei
AU - Lan, Jing
AU - Dang, Kuo
AU - Wang, Yanli
AU - Li, Dongbing
AU - Pan, Daodong
AU - Du, Lihui
AU - Dang, Yali
AU - Gao, Xinchang
N1 - Publisher Copyright:
© 2025 Elsevier Ltd
PY - 2025/12
Y1 - 2025/12
N2 - Caprine and bovine milk have been widely used as a supplementary nutritional source during early infancy. This study established an integrated peptidomics workflow combining in vitro digestion with multidimensional mass spectrometry (UHPLC-Q-TOF-MS/QqQ-MS) to decode species-specific peptide release patterns. Systemic peptidomic profiling identified 1728 and 1088 unique peptides in caprine and bovine skimmed milk digests, respectively, with β-casein-derived peptides dominating both systems (CHMP:126 vs BTMP:116). Two β-casein regions (P90-K112 and M159-L180) were identified as proteolytic hotspots generating structurally diverse peptides. Through relative-to-absolute quantification cascades, ten signature sequences were validated: LYQEPVLGPVR, SDIPNPIGSENSGK, YQEPVLGPVR, INNQFLPYPY, YPVEPFTESQSL, QGPIVLNPW, SLAMAASDISLL, LTEEEK, DMPIQAF, VLVLDTDYK, with species-dependent abundance: YQEPVLGPVR peaked in BTMP (0.84 mg·g−1) versus DMPIQAF in CHMP (1.39 mg·g−1). These sequence-resolved quantitative maps reveal the species-specific peptide clusters and provided templates for designing hypoallergenic infant formulas or cardiovascular nutraceuticals. This study provided data support for the functional research of dairy products in vivo.
AB - Caprine and bovine milk have been widely used as a supplementary nutritional source during early infancy. This study established an integrated peptidomics workflow combining in vitro digestion with multidimensional mass spectrometry (UHPLC-Q-TOF-MS/QqQ-MS) to decode species-specific peptide release patterns. Systemic peptidomic profiling identified 1728 and 1088 unique peptides in caprine and bovine skimmed milk digests, respectively, with β-casein-derived peptides dominating both systems (CHMP:126 vs BTMP:116). Two β-casein regions (P90-K112 and M159-L180) were identified as proteolytic hotspots generating structurally diverse peptides. Through relative-to-absolute quantification cascades, ten signature sequences were validated: LYQEPVLGPVR, SDIPNPIGSENSGK, YQEPVLGPVR, INNQFLPYPY, YPVEPFTESQSL, QGPIVLNPW, SLAMAASDISLL, LTEEEK, DMPIQAF, VLVLDTDYK, with species-dependent abundance: YQEPVLGPVR peaked in BTMP (0.84 mg·g−1) versus DMPIQAF in CHMP (1.39 mg·g−1). These sequence-resolved quantitative maps reveal the species-specific peptide clusters and provided templates for designing hypoallergenic infant formulas or cardiovascular nutraceuticals. This study provided data support for the functional research of dairy products in vivo.
KW - Simulated in vitro digestion
KW - Peptide quantification
KW - Protein traceability
UR - https://www.scopus.com/pages/publications/105012982227
U2 - 10.1016/j.foodres.2025.117264
DO - 10.1016/j.foodres.2025.117264
M3 - Article
AN - SCOPUS:105012982227
SN - 0963-9969
VL - 221
JO - Food Research International
JF - Food Research International
IS - part 1
M1 - 117264
ER -