Purification of factor X by hydrophobic interaction chromatography

Holger Husi; Malcolm D. Walkinshaw

doi:10.1016/S0378-4347(01)00112-8

Purification of factor X by hydrophobic interaction chromatography

Holger Husi^*
, Malcolm D. Walkinshaw

^*Corresponding author for this work

Research output: Journal Publication › Article › peer-review

5 Citations (Scopus)

Abstract

Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2-3M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available.

Original language	English
Pages (from-to)	367-371
Number of pages	5
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	755
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-4347(01)00112-8
Publication status	Published - 5 May 2001
Externally published	Yes

Free Keywords

Factor X
Purification

ASJC Scopus subject areas

General Chemistry

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10.1016/S0378-4347(01)00112-8

Cite this

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title = "Purification of factor X by hydrophobic interaction chromatography",

abstract = "Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2-3M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available.",

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AU - Walkinshaw, Malcolm D.

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AB - Human factor X has been purified to homogeneity by hydrophobic interaction chromatography on phenyl-sepharose. The coagulation protein did not interact with the resin in the presence of 2-3M NaCl whereas contaminants were retained. This single purification step, in conjunction with classical purification strategies, is a powerful tool in generating high purity factor X and is based on resins which are readily available.

KW - Factor X

KW - Purification

UR - https://www.scopus.com/pages/publications/0035810731

U2 - 10.1016/S0378-4347(01)00112-8

DO - 10.1016/S0378-4347(01)00112-8

M3 - Article

C2 - 11393727

AN - SCOPUS:0035810731

SN - 1387-2273

VL - 755

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JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

IS - 1-2

ER -

Purification of factor X by hydrophobic interaction chromatography

Abstract

Free Keywords

ASJC Scopus subject areas

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