TY - JOUR
T1 - Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity
T2 - Evidence from in vitro and in vivo tests
AU - Belhaj, Dalel
AU - Athmouni, Khaled
AU - Ahmed, Mohammad Boshir
AU - Aoiadni, Nissaf
AU - El Feki, Abdelfattah
AU - Zhou, John L.
AU - Ayadi, Habib
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.
AB - The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.
KW - Antioxidant defense system
KW - Cadmium exposure
KW - HepG2 cells
KW - P. versicolor polysaccharides
KW - Rat liver
UR - https://www.scopus.com/pages/publications/85043401795
U2 - 10.1016/j.ijbiomac.2018.02.152
DO - 10.1016/j.ijbiomac.2018.02.152
M3 - Article
C2 - 29499265
AN - SCOPUS:85043401795
SN - 0141-8130
VL - 113
SP - 813
EP - 820
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -