Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Dalel Belhaj; Khaled Athmouni; Mohammad Boshir Ahmed; Nissaf Aoiadni; Abdelfattah El Feki; John L. Zhou; Habib Ayadi

doi:10.1016/j.ijbiomac.2018.02.152

Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Dalel Belhaj^*
, Khaled Athmouni
, Mohammad Boshir Ahmed
, Nissaf Aoiadni
, Abdelfattah El Feki
, John L. Zhou
, Habib Ayadi

^*Corresponding author for this work

Research output: Journal Publication › Article › peer-review

28 Citations (Scopus)

Abstract

The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL⁻¹) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.

Original language	English
Pages (from-to)	813-820
Number of pages	8
Journal	International Journal of Biological Macromolecules
Volume	113
DOIs	https://doi.org/10.1016/j.ijbiomac.2018.02.152
Publication status	Published - 1 Jul 2018
Externally published	Yes

Free Keywords

Antioxidant defense system
Cadmium exposure
HepG2 cells
P. versicolor polysaccharides
Rat liver

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1016/j.ijbiomac.2018.02.152

Cite this

Belhaj, D., Athmouni, K., Ahmed, M. B., Aoiadni, N., El Feki, A., Zhou, J. L., & Ayadi, H. (2018). Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests. International Journal of Biological Macromolecules, 113, 813-820. https://doi.org/10.1016/j.ijbiomac.2018.02.152

@article{81cfb1205a884456b6ed558761b33363,

title = "Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests",

abstract = "The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.",

keywords = "Antioxidant defense system, Cadmium exposure, HepG2 cells, P. versicolor polysaccharides, Rat liver",

author = "Dalel Belhaj and Khaled Athmouni and Ahmed, \{Mohammad Boshir\} and Nissaf Aoiadni and \{El Feki\}, Abdelfattah and Zhou, \{John L.\} and Habib Ayadi",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

year = "2018",

month = jul,

day = "1",

doi = "10.1016/j.ijbiomac.2018.02.152",

language = "English",

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Belhaj, D, Athmouni, K, Ahmed, MB, Aoiadni, N, El Feki, A, Zhou, JL & Ayadi, H 2018, 'Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests', International Journal of Biological Macromolecules, vol. 113, pp. 813-820. https://doi.org/10.1016/j.ijbiomac.2018.02.152

Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests. / Belhaj, Dalel; Athmouni, Khaled; Ahmed, Mohammad Boshir et al.
In: International Journal of Biological Macromolecules, Vol. 113, 01.07.2018, p. 813-820.

Research output: Journal Publication › Article › peer-review

TY - JOUR

T1 - Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity

T2 - Evidence from in vitro and in vivo tests

AU - Belhaj, Dalel

AU - Athmouni, Khaled

AU - Ahmed, Mohammad Boshir

AU - Aoiadni, Nissaf

AU - El Feki, Abdelfattah

AU - Zhou, John L.

AU - Ayadi, Habib

PY - 2018/7/1

Y1 - 2018/7/1

N2 - The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.

AB - The in vitro antioxidant, cytotoxic and cytoprotective properties and in vivo hepatoprotective activities of crude polysaccharides extracted from cyanobacteria Phormidim versicolor NCC466 (CFv-PS) were investigated. The CFv-PS, identified as heteropolysaccharides with molecular weight of 63.79 kDa, exhibited relatively strong antioxidant activity, in a concentration-depended manner, in vitro assays. Additionally, CFv-PS did not induce cytotoxic effect on HepG2 human hepatocellular carcinoma cells within the range of tested concentrations (25–150 μg·mL−1) while preventing them against Cd. Moreover, in rats subjected to Cd-induced hepatotoxicity, CFv-PS pretreatment significantly (P < 0.05) reduced the level of ALAT, ASAT, biliburin, MDA, protein carbonyl and DNA damage, and markedly increased enzyme activities in liver tissues. These findings suggest that the cyanobacteria Phormidium versicolor is a potential source of natural products possessing antioxidant, cytoprotective and hepatoprotective properties.

KW - Antioxidant defense system

KW - Cadmium exposure

KW - HepG2 cells

KW - P. versicolor polysaccharides

KW - Rat liver

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VL - 113

SP - 813

EP - 820

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Belhaj D, Athmouni K, Ahmed MB, Aoiadni N, El Feki A, Zhou JL et al. Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests. International Journal of Biological Macromolecules. 2018 Jul 1;113:813-820. doi: 10.1016/j.ijbiomac.2018.02.152

Polysaccharides from Phormidium versicolor (NCC466) protecting HepG2 human hepatocellular carcinoma cells and rat liver tissues from cadmium toxicity: Evidence from in vitro and in vivo tests

Abstract

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