Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

A novel actinobacterial strain, designated MUSC 201^T, was isolated from a mangrove soil collected from Kuantan, the capital city of Pahang State in Malaysia. The taxonomic status of this strain was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis revealed that strain MUSC 201^T represented a novel lineage within the class Actinobacteria. Strain MUSC 201^T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 %), Aeromicrobium (92.7-94.6 %), Marmoricola (92.5-93.1 %) and Kribbella (91.5-92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5: 0.9: 1.0: 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H₄). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C_{18: 1}ω9c (30.8 %), C_{16: 0} (24.1 %), and 10- methyl C_{18: 0} (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed. The type strain of Mumia flava is MUSC 201^T (=DSM 27763^T=MCCC 1A00646_T=NBRC 109973^T).