TY - JOUR
T1 - Magnetic MOF-Based Immunosensing Strategy with Quantum Dot Labeling for Efficient Electro-chemiluminescent Detection of Alpha-fetoprotein
AU - Fatima, Mariyam
AU - Faizan, Muhammad
AU - Jabeen, Iqra
AU - Fiaz, Muhammad
AU - Ali, Ansar
AU - Ali, Syed Kashif
AU - Bahadur, Ali
AU - Iqbal, Shahid
AU - Mahmood, Sajid
AU - Jafri, Ibrahim
AU - Farouk, Abd Elaziem
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
PY - 2025
Y1 - 2025
N2 - This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab1). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab2) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples.
AB - This study presents the development of a highly sensitive and selective electrochemiluminescence (ECL) sandwich-type immunosensor for the detection of alpha-fetoprotein (AFP), a critical biomarker for early-stage liver cancer diagnosis. The sensor platform integrates a Fe-Ni-based metal-organic framework (MOF) with magnetic Fe₃O₄ nanoparticles (Fe-Ni-MOF), functionalized with primary antibodies (Ab1). Carbon quantum dots (CQDs) were conjugated with secondary antibodies (Ab2) to serve as ECL luminophores. The immunosensor demonstrated a wide linear detection range for AFP from 0.001 to 1000 ng/mL, with a low detection limit of 0.0084 ng/mL. Structural, morphological, thermal, and optical characterizations confirmed the successful synthesis and functionalization of the composite materials. Moreover, the fabricated sensor exhibited excellent reproducibility, specificity, and stability, indicating its potential as a practical tool for early cancer diagnostics in complex biological samples.
KW - Alpha-fetoprotein
KW - Biomarker
KW - Electrochemiluminescence
KW - Metal-organic framework
UR - https://www.scopus.com/pages/publications/105020790168
U2 - 10.1007/s10895-025-04616-0
DO - 10.1007/s10895-025-04616-0
M3 - Article
AN - SCOPUS:105020790168
SN - 1053-0509
JO - Journal of Fluorescence
JF - Journal of Fluorescence
ER -
