Landscape of neutralizing assessment of monoclonal antibodies against dengue virus

Jing Sun; Guang Lan Zhang; Lars Rønn Olsen; Ellis L. Reinherz; Vladimir Brusic

doi:10.1145/2506583.2512359

Landscape of neutralizing assessment of monoclonal antibodies against dengue virus

Jing Sun
, Guang Lan Zhang
, Lars Rønn Olsen
, Ellis L. Reinherz
, Vladimir Brusic

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

2 Citations (Scopus)

Abstract

The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.

Original language	English
Title of host publication	2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
Pages	836-842
Number of pages	7
DOIs	https://doi.org/10.1145/2506583.2512359
Publication status	Published - 2013
Externally published	Yes
Event	2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013 - Wshington, DC, United States Duration: 22 Sept 2013 → 25 Sept 2013

Publication series

Name	2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013

Conference

Conference	2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013
Country/Territory	United States
City	Wshington, DC
Period	22/09/13 → 25/09/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Free Keywords

B-cell epitope
Cross-reactivity
Dengue virus
Discontinuous peptide
Neutralizing antibody

ASJC Scopus subject areas

Bioengineering
Biomedical Engineering
Health Informatics

Access to Document

10.1145/2506583.2512359

Cite this

Sun, J., Zhang, G. L., Olsen, L. R., Reinherz, E. L., & Brusic, V. (2013). Landscape of neutralizing assessment of monoclonal antibodies against dengue virus. In 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013 (pp. 836-842). (2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013). https://doi.org/10.1145/2506583.2512359

@inproceedings{1a158702174644e0aa790aee1669a4e7,

title = "Landscape of neutralizing assessment of monoclonal antibodies against dengue virus",

abstract = "The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.",

keywords = "B-cell epitope, Cross-reactivity, Dengue virus, Discontinuous peptide, Neutralizing antibody",

author = "Jing Sun and Zhang, \{Guang Lan\} and Olsen, \{Lars R{\o}nn\} and Reinherz, \{Ellis L.\} and Vladimir Brusic",

year = "2013",

doi = "10.1145/2506583.2512359",

language = "English",

isbn = "9781450324342",

series = "2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013",

pages = "836--842",

booktitle = "2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013",

note = "2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013 ; Conference date: 22-09-2013 Through 25-09-2013",

}

Sun, J, Zhang, GL, Olsen, LR, Reinherz, EL & Brusic, V 2013, Landscape of neutralizing assessment of monoclonal antibodies against dengue virus. in 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013. 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013, pp. 836-842, 2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013, Wshington, DC, United States, 22/09/13. https://doi.org/10.1145/2506583.2512359

Landscape of neutralizing assessment of monoclonal antibodies against dengue virus. / Sun, Jing; Zhang, Guang Lan; Olsen, Lars Rønn et al.
2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013. 2013. p. 836-842 (2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013).

Research output: Chapter in Book/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Landscape of neutralizing assessment of monoclonal antibodies against dengue virus

AU - Sun, Jing

AU - Zhang, Guang Lan

AU - Olsen, Lars Rønn

AU - Reinherz, Ellis L.

AU - Brusic, Vladimir

PY - 2013

Y1 - 2013

N2 - The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.

AB - The majority of antibody binding sites (B-cell epitopes) on antigens are discontinuous. The binding between antigen and antibody is specific, but in some cases, the antibody elicited by one antigen will show cross-reactivity against other antigens. We have developed a bioinformatics-based approach for the analysis of sequence variability of neutralizing antibody binding sites and the assessment of coverage by individual neutralizing antibodies. The antigenic analysis of functional sites on the envelope (E) protein from dengue virus has been used as a case study. The description of B-cell epitopes, measurement of epitope similarity among different strains, and estimation of antibody neutralizing coverage provide insights in antibody cross-reactivity. We have defined a generalized method for the analysis of cross-reactivity of neutralizing antibodies that is also applicable to the analysis of other pathogens. This method adds to the toolset available for the characterization and the design of broadly neutralizing vaccines.

KW - B-cell epitope

KW - Cross-reactivity

KW - Dengue virus

KW - Discontinuous peptide

KW - Neutralizing antibody

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DO - 10.1145/2506583.2512359

M3 - Conference contribution

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SN - 9781450324342

T3 - 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013

SP - 836

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BT - 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013

T2 - 2013 4th ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013

Y2 - 22 September 2013 through 25 September 2013

ER -

Sun J, Zhang GL, Olsen LR, Reinherz EL, Brusic V. Landscape of neutralizing assessment of monoclonal antibodies against dengue virus. In 2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013. 2013. p. 836-842. (2013 ACM Conference on Bioinformatics, Computational Biology and Biomedical Informatics, ACM-BCB 2013). doi: 10.1145/2506583.2512359

Landscape of neutralizing assessment of monoclonal antibodies against dengue virus

Abstract

Publication series

Conference

UN SDGs

Free Keywords

ASJC Scopus subject areas

Access to Document

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Cite this