Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

Ma Bao-Kai; Li Mian; Cheong Ling-Zhi; Weng Xin-Chu; Shen Cai; Huang Qing

doi:10.15541/JIM20190139

Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

Ma Bao-Kai, Li Mian, Cheong Ling-Zhi, Weng Xin-Chu, Shen Cai, Huang Qing

Research output: Journal Publication › Article › peer-review

Abstract

Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.

Original language	English
Pages (from-to)	131-138
Number of pages	8
Journal	Journal of Inorganic Materials
Volume	35
Issue number	1
DOIs	https://doi.org/10.15541/JIM20190139
Publication status	Published - 2020
Externally published	Yes

Keywords

horse radish peroxidase
MXene nanosheets
biosensor
hydrogen peroxide

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10.15541/JIM20190139

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title = "Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2",

abstract = "Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.",

keywords = "horse radish peroxidase, MXene nanosheets, biosensor, hydrogen peroxide",

author = "Ma Bao-Kai and Li Mian and Cheong Ling-Zhi and Weng Xin-Chu and Shen Cai and Huang Qing",

year = "2020",

doi = "10.15541/JIM20190139",

language = "English",

volume = "35",

pages = "131--138",

journal = "Journal of Inorganic Materials",

number = "1",

}

TY - JOUR

T1 - Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

AU - Bao-Kai, Ma

AU - Mian, Li

AU - Ling-Zhi, Cheong

AU - Xin-Chu, Weng

AU - Cai, Shen

AU - Qing, Huang

PY - 2020

Y1 - 2020

N2 - Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.

AB - Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food.

KW - horse radish peroxidase

KW - MXene nanosheets

KW - biosensor

KW - hydrogen peroxide

UR - https://publons.com/publon/21354756/

U2 - 10.15541/JIM20190139

DO - 10.15541/JIM20190139

M3 - Article

VL - 35

SP - 131

EP - 138

JO - Journal of Inorganic Materials

JF - Journal of Inorganic Materials

IS - 1

ER -

Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2

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