TY - JOUR
T1 - Electrofermentation increases concentration of poly γ-glutamic acid in Bacillus subtilis biofilms
AU - Adilkhanova, Alina
AU - Ormantayeva, Anar
AU - Kaziullayeva, Aisholpan
AU - Olaifa, Kayode
AU - Eghtesadi, Neda
AU - Abbas, Azza H.
AU - Calvio, Cinzia
AU - Pham, Tri T.
AU - Ajunwa, Obinna M.
AU - Marsili, Enrico
N1 - Publisher Copyright:
© 2024 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd.
PY - 2024/3
Y1 - 2024/3
N2 - Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L−1), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L−1). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L−1. These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.
AB - Fluctuations in redox conditions in bioprocesses can alter the end-products, reduce their concentration, and lengthen the process time. Electrofermentation enables rapid metabolic modulation of biosynthesis and allows control of redox imbalances in biofilm-based fermentation processes. In this study, electrofermentation is used to boost the production of the bacterial biopolymer poly-γ-glutamic acid (γ-PGA) from Bacillus subtilis ATCC 6051. When compared to control experiments (3.3 ± 0.99 g L−1), the application of an electrode potential E = 0.4 V versus Ag/AgCl results in a more than two-fold increase in the production of γ-PGA (9.13 ± 1.4 g L−1). Using an engineered B. subtilis strain, in which γ-PGA production is driven by isopropyl β-d-1-thiogalactopyranoside, electrofermentation improves polymer concentrations from 15.4 ± 1.5 to 23.1 ± 1.6 versus g L−1. These results confirm that electrofermentation conditions can be adopted to increase the concentration of γ-PGA and perhaps other extracellular biopolymers in industrial strains.
UR - https://www.scopus.com/pages/publications/85188045722
U2 - 10.1111/1751-7915.14426
DO - 10.1111/1751-7915.14426
M3 - Article
C2 - 38497275
AN - SCOPUS:85188045722
SN - 1751-7907
VL - 17
JO - Microbial Biotechnology
JF - Microbial Biotechnology
IS - 3
M1 - e14426
ER -