Bioaccumulation of sulfadiazine and subsequent enzymatic activities in Chinese mitten crab (Eriocheir sinensis)

The bioaccumulation of sulfadiazine and subsequent enzymatic activities in Chinese mitten crab (Eriocheir sinensis) were studied in microcosms, by exposing to 50, 100, 500 and 1000 ng/L of sulfadiazine for 44 days. An effective method for extracting sulfadiazine in crab tissues was established by modifying the cleanup method after ultrasound extraction, with improved recoveries of 61.8%, 93.7% and 100.5% in gill, muscle and liver samples. The results showed that sulfadiazine residues were all < 3 ng/g dry weight in different tissues, and that sulfadiazine bioaccumulation in crab was not dose-dependent. A significantly negative correlation was observed between acetylcholinesterase activity and the residue concentration of sulfadiazine during exposure to 50 ng/L and 1000 ng/L, and between alkaline phosphatase and sulfadiazine residues in the 100 ng/L exposure group in the gill, suggesting that the two enzymes played an important role in the metabolism of sulfadiazine in crab.