Barrientosiimonas humi gen. nov., sp. nov., an actinobacterium of the family Dermacoccaceae

Three novel actinobacteria, strains 39^T, 40 and 41, were isolated from soil collected from Barrientos Island in the Antarctic. The taxonomic status of these strains was determined using a polyphasic approach. Comparison of 16S rRNA gene sequences revealed that strain 39^T represented a novel lineage within the family Dermacoccaceae and was most closely related to members of the genera Demetria (96.9% 16S rRNA gene sequence similarity), Branchiibius (95.7%), Dermacoccus (94.4-95.3%), Calidifontibacter (94.6%), Luteipulveratus (94.3%), Yimella (94.2%) and Kytococcus (93.1%). Cells were irregular cocci and short rods. The peptidoglycan type was A4a with an L-Lys-L-Ser-D-Asp interpeptide bridge. The cell-wall sugars were galactose and glucose. The major menaquinone was MK-8(H₄). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphoglycolipid, two glycolipids and one unknown phospholipid. The acyl type of the cell-wall polysaccharide was N-acetyl. The major cellular fatty acids were anteiso-C_{17: 0} (41.97%), anteiso-C_{17: 1}ω9c (32.16%) and iso-C_{16: 0} (7.68%). The DNA G+C content of strain 39^T was 68.4 mol%. On the basis of phylogenetic and phenotypic differences from other genera of the family Dermacoccaceae, a novel genus and species, Barrientosiimonas humi gen. nov., sp. nov., is proposed; the type strain of the type species is 39^T (=CGMCC 4.6864^T =DSM 24617^T).